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KMID : 1007519950040020061
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Food Science and Biotechnology
1995 Volume.4 No. 2 p.61 ~ p.65
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Determination of Lactose in Milks by Multiple Enzyme E1ectrode
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Park, In-Seon
Kim, Jung-Ho/Kim, Tai-Jin/Noh, Bong-Soo
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Abstract
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An lactose-sensing electrode based on a tri-enzyme system was prepared. Asymmetric coupling on a nylon net was used for the immobilization of Pgalactosidase and mutarotase. Glucose oxidase was chemically immobilized on the nylon net that was placed over an oxygen electrode. The optimum conditions for the lactose sensor were found to be 0.1M potassium phosphate buffer (pH 7.5) at 35℃. The calibration curve for lactose was linear in the range between 0.0625~4 g/100 ml. The lactose sensor exhibited a short response time (35~45 sec) based on the maximum rate of oxygen consumption. Glucose, sucrose and oligosaccharide interfered in the measurement of lactose, but there was no interference in milk or skim milk. Lactose in milk was determined by a multiple enzyme electrode. It was in the range of 4.85~5.79 g/L. The tri-enzyme sensor system was easy and simple compared to spectrometry with an enzyme test-kit.
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